Biofilm is a defensive effort in which colonies of similar bacteria protect themselves against chemical assault. The germs cooperate to build and maintain a barrier that keeps even strong disinfectants such as bleach and iodine at bay.
Getting rid of biofilm is a two-step process. Mechanical action, coupled with the use of disinfectant, is the simplest, most inexpensive method of breaking down biofilm and killing the underlying bacteria. A brush or nylon scouring pad provides the agitation necessary to break up the film so that the disinfectant can do its job.
A common place in the home for biofilm formation is under the rims of toilets. That’s why a toilet brush, toilet swab and a disinfectant-detergent are valuable weapons in keeping the bowl clean. To do the job right, first use a toilet brush or nylon scouring pad to break up the film, and then apply disinfectant with a toilet swab to kill the underlying bacteria.
Biofilms can be present even when the surface doesn't feel or look slimy, however. Biofilms may be removed by physical agitation such as brushing or scrubbing.
For more information, see the Housekeeping Channel article, “Are you Winning the Battle with Biofilm?”